Genetic Information

Gene & Transcript Details

Gene
ATM
Transcript
NM_000051.4 MANE Select
Total Exons
Reference Sequence
NC_000011.9
Alternative Transcripts
IDStatusDetails
NM_000051.3 RefSeq Select 13147 nt | 386–9556
NM_000051.4 MANE Select 12915 nt | 151–9321

Variant Details

HGVS Notation
NM_000051.4:c.8763G>T
Protein Change
T2921=
Location
Exon 60 (Exon 60 of )
60
5'Exon Structure3'
Functional Consequence
Loss of Function
Alternate Identifiers

Clinical & Population Data

Population Frequency

gnomAD
Global Frequency
0.000398 in 100,000
Extremely Rare
ACMG Criteria Applied PM2
This variant is absent or extremely rare in population databases (PM2 criteria applies).

ClinVar

Open
Classification
Likely Benign
2 publications
Clinical Statement

"This variant has been reported in ClinVar as Likely benign (4 clinical laboratories) and as Benign (1 clinical laboratories)."

COSMIC Somatic Evidence

Open
COSMIC ID
Recurrence
0 occurrences
PM1 Criteria
Not Applied
COSMIC Database Preview
COSMIC Preview
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Functional Impact & Domains

Functional Domain

Hotspot Status
Not a hotspot
Domain Summary
This variant is not located in a mutational hotspot or critical domain.
Related Variants in This Domain
No evidence of other pathogenic variants at this position in gene ATM.

Functional Studies & Therapeutic Relevance

Functional Summary

The variant has not been functionally characterized.

Database Previews
OncoKB
OncoKB Preview
JAX-CKB
JAX-CKB Preview

Click on previews to view full database entries. External databases may require institutional access.

Computational Analysis

Pathogenicity Predictions

SpliceAI
Predictor Consensus
Mixed/VUS
PP3 Applied
No
REVEL Score
0.0
Threshold: ≥0.75 = PP3 applied

SpliceAI Scores

Window: ±500bp
Effect Type Score Position
- Acceptor Loss (AL) 0.0 -46 bp
- Donor Loss (DL) 0.0 -74 bp
+ Acceptor Gain (AG) 0.0 -91 bp
+ Donor Gain (DG) 0.0 -196 bp
High impact (≥0.5) Medium impact (0.2-0.49) Low impact (<0.2)

VCEP Guidelines

Applied ACMG/AMP Criteria (VCEP Specific)

Filter Criteria:
PVS1

PVS1 (Not Applied)

According to VCEP guidelines, the rule for PVS1 is: "Use ATM PVS1 Decision Tree to evaluate null variants (nonsense, frameshift, canonical ±1–2 splice sites, initiation codon, single exon or multiexon deletion)". The evidence for this variant shows: it is a synonymous (silent) change with no predicted impact on splicing. Therefore, this criterion is not applied because the variant does not result in a null allele.

PS1

PS1 (Not Applied)

According to VCEP guidelines, the rule for PS1 is: "Same amino acid change as a known pathogenic variant (with different nucleotide change), provided splicing is ruled out". The evidence for this variant shows: there is no amino acid change (T2921=). Therefore, this criterion is not applied.

PS2

PS2 (Not Applied)

According to standard ACMG guidelines, the rule for PS2 is: "De novo (both maternity and paternity confirmed) in a patient with the disease and no family history". The evidence for this variant shows: no de novo segregation data are available. Therefore, this criterion is not applied.

PS3

PS3 (Not Applied)

According to VCEP guidelines, the rule for PS3 is: "Use functional studies showing a deleterious effect on ATM-specific assays (e.g., failure to rescue kinase activity and radiosensitivity)". The evidence for this variant shows: no functional characterization has been performed. Therefore, this criterion is not applied.

PS4

PS4 (Not Applied)

According to standard ACMG guidelines, the rule for PS4 is: "Prevalence in affected individuals significantly increased compared to controls (case–control data with p≤0.05 AND OR≥2 or lower CI≥1.5)". The evidence for this variant shows: no case–control or statistical association data. Therefore, this criterion is not applied.

PM1

PM1 (Not Applied)

According to standard ACMG guidelines, the rule for PM1 is: "Located in a mutational hot spot or well‐studied functional domain without benign variation". The evidence for this variant shows: it is synonymous and not within a defined critical domain. Therefore, this criterion is not applied.

PM2

PM2 (Supporting)

According to VCEP guidelines, the rule for PM2_supporting is: "Frequency ≤.001% if n=1 in a single subpopulation, that is sufficiently rare and PM2_supporting would apply". The evidence for this variant shows: global gnomAD frequency is 0.000398% with allele count = 1. Therefore, this criterion is applied at Supporting strength because the variant meets the gene‐specific rarity threshold.

PM3

PM3 (Not Applied)

According to VCEP guidelines, the rule for PM3 is: "Observed in trans with a pathogenic variant in a recessive disorder (using ATM PM3/BP2 table)". The evidence for this variant shows: no data on in trans observations. Therefore, this criterion is not applied.

PM4

PM4 (Not Applied)

According to standard ACMG guidelines, the rule for PM4 is: "Protein length changes due to in‐frame indels or stop‐loss variants". The evidence for this variant shows: no change in protein length. Therefore, this criterion is not applied.

PM5

PM5 (Not Applied)

According to VCEP guidelines, the rule for PM5_supporting is: "Novel missense change at an amino acid residue where a different pathogenic missense change has been seen". The evidence for this variant shows: no amino acid change. Therefore, this criterion is not applied.

PM6

PM6 (Not Applied)

According to standard ACMG guidelines, the rule for PM6 is: "Assumed de novo, but without confirmation of paternity and maternity". The evidence for this variant shows: no de novo data. Therefore, this criterion is not applied.

PP1

PP1 (Not Applied)

According to standard ACMG guidelines, the rule for PP1 is: "Co‐segregation with disease in multiple affected family members". The evidence for this variant shows: no segregation data. Therefore, this criterion is not applied.

PP2

PP2 (Not Applied)

According to standard ACMG guidelines, the rule for PP2 is: "Missense variant in a gene with low rate of benign missense variation and where missense is a common mechanism". The evidence for this variant shows: it is synonymous, not missense. Therefore, this criterion is not applied.

PP3

PP3 (Not Applied)

According to VCEP guidelines, the rule for PP3 is: "REVEL >.733 or RNA predictor shows splice impact". The evidence for this variant shows: SpliceAI score = 0 (no splice impact) and no REVEL data to suggest deleterious effect. Therefore, this criterion is not applied.

PP4

PP4 (Not Applied)

According to standard ACMG guidelines, the rule for PP4 is: "Patient’s phenotype or family history is highly specific for a disease with a single genetic etiology". The evidence for this variant shows: no phenotype data. Therefore, this criterion is not applied.

PP5

PP5 (Not Applied)

According to standard ACMG guidelines, the rule for PP5 is: "Reputable source reports pathogenic but evidence unavailable". The evidence for this variant shows: reputable sources report benign/likely benign, not pathogenic. Therefore, this criterion is not applied.

BA1

BA1 (Not Applied)

According to VCEP guidelines, the rule for BA1 is: "Filtering allele frequency >.5%". The evidence for this variant shows: global frequency = 0.000398%, well below 0.5%. Therefore, this criterion is not applied.

BS1

BS1 (Not Applied)

According to VCEP guidelines, the rule for BS1 is: "Filtering allele frequency >.05%". The evidence for this variant shows: global frequency = 0.000398%, below 0.05%. Therefore, this criterion is not applied.

BS2

BS2 (Not Applied)

According to standard ACMG guidelines, the rule for BS2 is: "Observed in healthy adult individuals for a fully penetrant dominant disorder". The evidence for this variant shows: no such observations reported. Therefore, this criterion is not applied.

BS3

BS3 (Not Applied)

According to VCEP guidelines, the rule for BS3 is: "Well‐established functional studies show no deleterious effect on ATM‐specific assays". The evidence for this variant shows: no functional rescue data. Therefore, this criterion is not applied.

BS4

BS4 (Not Applied)

According to standard ACMG guidelines, the rule for BS4 is: "Lack of segregation in affected members of a family". The evidence for this variant shows: no segregation data. Therefore, this criterion is not applied.

BP1

BP1 (Not Applied)

According to standard ACMG guidelines, the rule for BP1 is: "Missense variant in a gene where only truncating variants cause disease". The evidence for this variant shows: it is synonymous. Therefore, this criterion is not applied.

BP2

BP2 (Not Applied)

According to VCEP guidelines, the rule for BP2 is: "Observed in cis with pathogenic variant in recessive disorder". The evidence for this variant shows: no data on cis/trans phase. Therefore, this criterion is not applied.

BP3

BP3 (Not Applied)

According to standard ACMG guidelines, the rule for BP3 is: "In-frame deletion/duplication in repetitive region". The evidence for this variant shows: not applicable (synonymous SNV). Therefore, this criterion is not applied.

BP4

BP4 (Not Applied)

According to VCEP guidelines, the rule for BP4 is: "REVEL ≤.249 or RNA predictor shows splice impact". The evidence for this variant shows: SpliceAI score = 0 (no splice impact) and no REVEL data. Therefore, this criterion is not applied.

BP5

BP5 (Not Applied)

According to standard ACMG guidelines, the rule for BP5 is: "Variant found in a case with an alternate molecular basis for disease". The evidence for this variant shows: no such alternate pathogenic basis reported. Therefore, this criterion is not applied.

BP6

BP6 (Supporting)

According to standard ACMG guidelines, the rule for BP6 is: "Reputable source reports variant as benign, but the evidence is not available to the laboratory". The evidence for this variant shows: ClinVar entries from five laboratories (four Likely benign, one Benign). Therefore, this criterion is applied at Supporting strength because reputable sources classify the variant benignly without available evidence.

BP7

BP7 (Supporting)

According to standard ACMG guidelines, the rule for BP7 is: "Synonymous variant with no predicted impact on splicing". The evidence for this variant shows: SpliceAI score = 0, indicating no impact on splicing. Therefore, this criterion is applied at Supporting strength.