NM_000267.3:c.2325+16C>G is an intronic variant located at position +16 in intron 19 of NF1. SpliceAI predicts no splicing impact (max delta score 0.02).¹ This variant is present in gnomAD population databases at very low frequency: 14 of 281,840 alleles in v2.1 (AF=0.005%) and 185 of 1,612,866 alleles in v4.1 (AF=0.011%). It is absent from gnomAD-Canada.² In ClinVar, this variant is classified as Likely benign (VariationID 512450) by 4 clinical laboratories including GeneDx, Athena Diagnostics, Genome-Nilou Lab, and Labcorp Genetics.³ No published literature was identified that directly mentions NM_000267.3:c.2325+16C>G. Eight papers from the ClinVar submission record were reviewed; all are general guidelines, methodology papers, or disease reviews that do not report this specific variant. The available evidence supports a likely benign interpretation: BP4 (SpliceAI predicts no splicing impact) and BP6 (ClinVar consensus of Likely benign from 4 clinical laboratories). No pathogenic criteria are met.⁴