NM_000546.6:c.-29+100dup is an intronic duplication at position +100 of intron 1 in TP53. SpliceAI predicts no splicing impact (max delta score = 0.00).¹ The variant is present in gnomAD v4.1 at an extremely low frequency (AF=2.26×10⁻⁵, 9/398,376 alleles; grpmax FAF=2.07×10⁻⁵), meeting PM2_Supporting per TP53 VCEP thresholds (total AF < 0.00003; subpopulation AF < 0.00004).² As an intronic variant outside the core splice motif with SpliceAI score ≤ 0.1, BP4_Supporting is met per the TP53 VCEP PP3/BP4/BP7 flowchart.³ As an intronic variant at position +100 (beyond +7) with SpliceAI predicting no splice impact, BP7_Supporting is met per the TP53 VCEP specification (Walker et al. 2023, PMID:37352859).⁴ The variant is absent from ClinVar and COSMIC and has not been reported in the literature. No proband, segregation, de novo, or functional data are available.⁵ All other ACMG/AMP criteria are either not applicable (intronic variant outside scope of VCEP rules for missense/null/functional criteria) or not assessed due to absence of clinical data.