NM_000535.7:c.705+24C>T is an intronic variant in PMS2 located at position +24 of intron 6, outside the canonical splice consensus region.¹ This variant is present in gnomAD v4.1 at an extremely low overall allele frequency of 1.18e-05 (19/1,610,524 alleles, no homozygotes), meeting PM2_Supporting per InSiGHT PMS2 VCEP criteria (allele frequency <0.00002).² SpliceAI predicts no splicing impact with a maximum delta score of 0.04, meeting BP4_Supporting per the InSiGHT PMS2 VCEP (delta ≤0.1 for intronic variants).³ The variant is at intronic position +24, which is beyond the +7 canonical splice donor boundary, meeting BP7_Supporting per the InSiGHT PMS2 VCEP (intronic variants at or beyond -21/+7).⁴ The variant is absent from ClinVar and no publications were identified that mention NM_000535.7:c.705+24C>T.⁵ The variant has been observed in COSMIC (COSV113734575, n=2 somatic occurrences), but this does not constitute germline evidence of pathogenicity. Criteria met: PM2_Supporting (extremely rare in population databases). Benign criteria met: BP4_Supporting (SpliceAI predicts no splice impact), BP7_Supporting (intronic variant beyond canonical splice region).⁶ Per InSiGHT PMS2 VCEP v2.0.0 combination Rule 31, the co-occurrence of pathogenic supporting evidence (PM2) and benign supporting evidence (BP4, BP7) results in Uncertain Significance — Conflicting Evidence. While the variant also satisfies Rule 19 (≥2 benign supporting criteria for Likely Benign), the presence of pathogenic supporting evidence triggers the conflicting evidence rule.⁷