The PTEN c.743del (p.(Pro248LeufsTer8)) variant has been observed in somatic cancers in COSMIC (COSV109436597, n=2) and has been reported in ClinVar as pathogenic.¹ This variant is absent from gnomAD v2.1 and gnomAD v4.1, which is below the PTEN PM2_Supporting threshold of 0.001% and supports rarity in the general population.² The variant is a frameshift predicted to truncate PTEN at codon 255, and under the PTEN Expert Panel PVS1 decision tree this position is 5' of the p.D375 threshold for full-strength PVS1 in transcript NM_000314.8.³ SpliceAI predicts no significant splice impact for this variant (maximum delta score 0.06), and this does not alter the interpretation of the variant as a loss-of-function frameshift.⁴