NM_007294.4:c.2531G>C (p.Ser844Thr) is a missense variant in BRCA1 exon 10, absent from gnomAD v2.1 and v4.1 population databases (PM2_Supporting).¹ The variant is located at residue 844, outside ENIGMA-defined clinically important functional domains (RING aa 2-101, coiled-coil aa 1391-1424, BRCT aa 1650-1857), and SpliceAI predicts no splicing impact (max delta = 0.00), meeting BP1_Strong.² No functional assay data are available for this variant in ENIGMA-calibrated studies (PS3/BS3 not met). The variant was searched in the ENIGMA Table 9 curated functional assay results, ST4 full functional dataset, ST5 mammalian functional studies, and Parsons et al. 2019 datasets and was not found in any.³ No clinical-history likelihood ratio is available from Li et al. 2020 (PMID:31853058) for this variant; c.2531G>C was not present in the BRCA1 clinical_history_LR table (PP4/BP5 not met).⁴ Under the ENIGMA point system for conflicting evidence (Table 3), PM2_Supporting (+1) and BP1_Strong (-4) sum to -3, which falls in the Likely Benign range (-6 to -2). The variant is classified as Likely Benign.⁵