NM_024642.5:c.-28C>T is a 5' UTR variant in GALNT12. It is present at extremely low frequency in gnomAD (v2.1 AF=0.00327%, v4.1 AF=0.00435%), meeting PM2 at supporting level.¹ SpliceAI predicts no splicing impact (max delta score = 0.00), but this single line of computational evidence is insufficient to meet PP3 or BP4 thresholds. REVEL and BayesDel scores are unavailable as the variant does not alter an amino acid.² This variant is absent from ClinVar and has not been reported in the published literature. No functional studies, case-control data, cosegregation data, or de novo observations are available.³ PVS1 is not applicable as c.-28C>T is a 5' UTR substitution rather than a null variant (nonsense, frameshift, canonical splice, initiation codon, or exon deletion) eligible under the ClinGen SVI framework.⁴ BS2 is not applicable for this gene: GALNT12 is a moderate-penetrance adult-onset cancer predisposition gene, and observation in healthy population controls does not constitute strong evidence against pathogenicity. The only criterion met is PM2 (supporting). All other assessed criteria are either not met, not assessed due to insufficient evidence, or not applicable. This yields a classification of Variant of Uncertain Significance (VUS) per ACMG/AMP 2015 generic rules.⁵