NM_001308632.1:c.319C>T (p.Pro107Ser) is a missense variant in exon 3 of POLD1. It is absent from gnomAD v2.1, v4.1, and gnomAD-Canada population databases (PM2_supporting).¹ Multiple in silico predictors suggest a benign effect: REVEL score 0.015, BayesDel score -0.70, and SpliceAI max delta 0.01 predict no deleterious impact on protein function or splicing (BP4_supporting).² This variant has been reported in ClinVar (Variation ID 848162) as a Variant of Uncertain Significance by two clinical laboratories (Labcorp, GeneDx) and as Likely benign by one laboratory (Ambry Genetics). No expert panel review has been performed.³ No variant-specific functional studies, segregation data, case-control analyses, or de novo reports were identified. OncoKB reports unknown oncogenic effect with no curated functional evidence.⁴ Under generic ACMG/AMP 2015 combination rules, one supporting pathogenic criterion (PM2) and one supporting benign criterion (BP4) result in an overall classification of Variant of Uncertain Significance (VUS).⁵