PS1
Not assessed: no ClinVar record or literature reports exist, so no same-amino-acid pathogenic comparator (p.Tyr2023Phe) was available.
PS2
Not assessed: no proband phenotype, family history, or parental genotyping data were available to evaluate a de novo origin.
PS3
Not assessed: no functional studies exist for this variant; OncoKB reports unknown oncogenic effect.
PS4
Not assessed: no case-control or cohort allele-frequency comparison exists for this variant.
PM1
Not met: no statistically significant hotspot at ROS1 Y2023, and no curated ROS1 domain map supports this position.
PM5
Not assessed: no pathogenic variant at the same residue (p.Tyr2023) was identified for comparison.
PM6
Not assessed: no de novo occurrence or parental testing results were reported for this variant.
PP1
Not assessed: no pedigree or family segregation data exist for this variant.
PP2
Not assessed: no ROS1 missense-constraint data (e.g., gnomAD Z-score) were available.
PP3
Not met: SpliceAI max delta 0.01 predicts no splice impact, and REVEL 0.52 is uninformative (below 0.773, above 0.016).
PP4
Not assessed: no carrier phenotype or clinical case description was available for this variant.
PP5
Not met: this variant has no ClinVar record, so no expert-panel pathogenic classification exists to support PP5.
