NM_000051.3:c.7527G>A (Met2509Ile) is a missense variant in ATM exon 51. It is absent from gnomAD v4.1 population databases (PM2_Supporting).¹ Multiple in silico computational tools predict a benign effect: REVEL score 0.184 (BP4 threshold ≤0.249); SpliceAI max delta 0.06 (no predicted splicing impact); BayesDel score -0.366 (BP4_Supporting).² The variant is classified as 'Non-functional' by computational meta-prediction in Suppl_TableS1 (PMID 40580951, combined score -1.87, medium-high confidence), but this is a computational prediction rather than experimental functional data and does not meet VCEP requirements for PS3 or BS3.³ This variant has been reported in ClinVar as Uncertain significance by two clinical laboratories and as Likely benign by one clinical laboratory (ClinVar Variation ID: 407522). Under VCEP v1.5, PP5 and BP6 are not applicable.⁴ No variant-specific literature was identified; seven ClinVar-associated PMIDs were reviewed and none mentioned NM_000051.3:c.7527G>A.⁵ Applying ATM VCEP v1.5 rules: PM2_Supporting (1 pathogenic supporting point) and BP4_Supporting (1 benign supporting point) result in a classification of Uncertain Significance per Rule 31 (conflicting evidence: ≥1 benign supporting + ≥1 pathogenic supporting).⁶