PS1
Not met: no established pathogenic variant produces the same p.Lys965Asn change; the only ClinVar entry is a 1-star VUS.
PS2
Not assessed: no de novo occurrence or parental testing for this variant is recorded in any source.
PS3
Not assessed: no functional studies exist; OncoKB reports no variant-specific evidence and COSMIC lacks the variant.
PS4
Not assessed: no case-control or case-series data exist for this variant in any retrieved source.
PM1
Not met: residue 965 shows no statistically significant cancer hotspot and no curated critical-domain annotation.
PM5
Not met: no pathogenic alternate missense at residue 965; the screen found zero same-residue candidates.
PM6
Not assessed: no de novo occurrence or parental genotypes are reported for this variant.
PP1
Not assessed: no co-segregation data — no affected relatives, pedigree, or segregation counts available.
PP2
Not assessed: missense is a known RET disease mechanism, but no gene-level benign-missense-rate data was available.
PP3
Not met: no in silico tool predicts damage — SpliceAI 0.00, BayesDel −0.186, REVEL 0.529 intermediate.
PP4
Not assessed: no proband phenotype or family history information was available for this variant.
PP5
Not met: no ClinVar expert-panel pathogenic assertion; the sole submission is a non-expert 1-star VUS.
